{"product_id":"14-15-eet-dhet-elisa-kit-5202026","title":"14,15-EET\/DHET ELISA Kit","description":"\u003cp\u003eThe 14,15-EET\/DHET ELISA Kit measures 14,15-EET\/DHET in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 14,15-EET\/DHET. 14,15-EET\/DHET in the sample or standard competes with a fixed amount of biotin-labeled 14,15-EET\/DHET for sites on a pre-coated antibody specific to 14,15-EET\/DHET. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of 14,15-EET\/DHET in the sample. The concentration of the target substance is inversely proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027951689956,"sku":"EU2642","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EU2642.jpg?v=1779907034","url":"https:\/\/www.ardentbio.com\/products\/14-15-eet-dhet-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}