{"product_id":"25oh-d-total-25-oh-vitamin-d-elisa-kit-5202026","title":"25OH-D (Total 25-OH Vitamin D) ELISA Kit","description":"\u003cp\u003eThe 25OH-D (Total 25-OH Vitamin D) ELISA Kit measures 25OH-D in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 25OH-D. 25OH-D in the sample or standard competes with a fixed amount of biotin-labeled 25OH-D for sites on a pre-coated antibody specific to 25OH-D. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of 25OH-D in the sample. The concentration of the target substance is inversely proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027952181476,"sku":"EU3593","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EU3593_8e08cc97-c682-4d65-b7b6-60ab32b94fc8.jpg?v=1779907042","url":"https:\/\/www.ardentbio.com\/products\/25oh-d-total-25-oh-vitamin-d-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}