{"product_id":"27-ohc-27-hydroxycholesterol-elisa-kit-5202026","title":"27-OHC (27-Hydroxycholesterol) ELISA Kit","description":"\u003cp\u003eThe 27-OHC (27-Hydroxycholesterol) ELISA Kit measures 27-OHC in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 27-OHC. 27-OHC in the sample or standard competes with a fixed amount of biotin-labeled 27-OHC for sites on a pre-coated antibody specific to 27-OHC. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of 27-OHC in the sample. The concentration of the target substance is inversely proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027952214244,"sku":"EU2632","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EU2632_323cc91a-66f5-4f8d-a88c-33917da6de94.jpg?v=1779907044","url":"https:\/\/www.ardentbio.com\/products\/27-ohc-27-hydroxycholesterol-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}