{"product_id":"baff-cd257-b-cell-activating-factor-elisa-kit-small-sample-4292026","title":"BAFF\/CD257 (B-Cell Activating Factor) ELISA Kit (Small Sample)","description":"\u003cp\u003eThe BAFF\/CD257 (B-Cell Activating Factor) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BAFF\/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BAFF\/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BAFF\/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BAFF\/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e","brand":"Elk Biotechnology","offers":[{"title":"96 wells","offer_id":48862801068260,"sku":"ELK2145MS","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 wells","offer_id":48862801101028,"sku":"ELK2145MS","price":416.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/ELK2145MS-b-1.jpg?v=1777675872","url":"https:\/\/www.ardentbio.com\/products\/baff-cd257-b-cell-activating-factor-elisa-kit-small-sample-4292026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}