{"product_id":"bovine-il1ra-interleukin-1-receptor-antagonist-elisa-kit-5202026","title":"Bovine IL1RA (Interleukin 1 Receptor Antagonist) ELISA Kit","description":"\u003cp\u003eThe Bovine IL1RA (Interleukin 1 Receptor Antagonist) ELISA Kit measures IL-1ra in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-1ra antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-1ra bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-1ra in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49020795945188,"sku":"EB0169","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EB0169_b06c205d-7b27-4255-8ce4-7ab25b1f140b.jpg?v=1779893634","url":"https:\/\/www.ardentbio.com\/products\/bovine-il1ra-interleukin-1-receptor-antagonist-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}