{"product_id":"canine-mmp2-matrix-metalloproteinase-2-elisa-kit-5202026","title":"Canine MMP2 (Matrix metalloproteinase-2) ELISA Kit","description":"\u003cp\u003eThe Canine MMP2 (Matrix metalloproteinase-2) ELISA Kit measures MMP-2 in Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti MMP-2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with MMP-2 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of MMP-2 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49020830417124,"sku":"ECA0047","price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/ECA0047_63ecfb47-54ad-4ed0-8e25-e003cae30dc9.jpg?v=1779893852","url":"https:\/\/www.ardentbio.com\/products\/canine-mmp2-matrix-metalloproteinase-2-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}