{"product_id":"equine-cox-2-cyclooxygenase-2-elisa-kit-5202026","title":"Equine COX-2 (Cyclooxygenase-2) ELISA Kit","description":"\u003cp\u003eThe Equine COX-2 (Cyclooxygenase-2) ELISA Kit measures PTGS2\/COX-2 in Equine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with COX-2. COX-2 in the sample or standard competes with a fixed amount of biotin-labeled COX-2 for sites on a pre-coated antibody specific to COX-2. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of COX-2 in the sample. The concentration of the target substance is inversely proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49020904898788,"sku":"EHS0065","price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EHS0065_8a869627-8c96-4d03-b324-39d7078f54ad.jpg?v=1779894263","url":"https:\/\/www.ardentbio.com\/products\/equine-cox-2-cyclooxygenase-2-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}