{"product_id":"guinea-pig-il-15-interleukin-15-elisa-kit-5202026","title":"Guinea pig IL-15 (Interleukin 15) ELISA Kit","description":"\u003cp\u003eThe Guinea pig IL-15 (Interleukin 15) ELISA Kit measures IL-15 in Guinea Pig Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-15 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-15 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-15 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49020881109220,"sku":"EGP0027","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EGP0027_2d9faa30-8b89-48d5-838a-4d5f508b56ad.jpg?v=1779894158","url":"https:\/\/www.ardentbio.com\/products\/guinea-pig-il-15-interleukin-15-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}