{"product_id":"hamster-tnf-alpha-tumor-necrosis-factor-alpha-elisa-kit-5202026","title":"Hamster TNF-Alpha (Tumor Necrosis Factor-Alpha) ELISA Kit","description":"\u003cp\u003eThe Hamster TNF-Alpha (Tumor Necrosis Factor-Alpha) ELISA Kit measures TNF-Alpha in Hamster Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TNF-α antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with TNF-α bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TNF-α in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49020901392612,"sku":"EHA0004","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EHA0004_95a021f6-1bf8-429c-808f-5f145bece559.jpg?v=1779894244","url":"https:\/\/www.ardentbio.com\/products\/hamster-tnf-alpha-tumor-necrosis-factor-alpha-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}