{"product_id":"hla-b27-leukocyte-antigen-b27-elisa-kit-small-sample-4292026","title":"HLA-B27 (Leukocyte Antigen B27) ELISA Kit (Small Sample)","description":"\u003cp\u003eThe HLA-B27 (Leukocyte Antigen B27) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HLA-B27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HLA-B27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HLA-B27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HLA-B27 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e","brand":"Elk Biotechnology","offers":[{"title":"96 wells","offer_id":48862895702244,"sku":"ELK0864MS","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 wells","offer_id":48862895735012,"sku":"ELK0864MS","price":416.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/ELK0864MS-b-1.jpg?v=1777675835","url":"https:\/\/www.ardentbio.com\/products\/hla-b27-leukocyte-antigen-b27-elisa-kit-small-sample-4292026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}