{"product_id":"human-5-hydroxytryptamine-elisa-kit-5202026","title":"Human 5-hydroxytryptamine ELISA Kit","description":"\u003cp\u003eThe Human 5-hydroxytryptamine ELISA Kit measures 5-HT in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 5-hydroxytryptamine. 5-hydroxytryptamine in the sample or standard competes with a fixed amount of biotin-labeled 5-hydroxytryptamine for sites on a pre-coated antibody specific to 5-hydroxytryptamine. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of 5-hydroxytryptamine in the sample. The concentration of the target substance is inversely proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49020911747300,"sku":"EH4005","price":455.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EH4005_7dafbfaa-5cc6-434e-9286-13ae502ef995.jpg?v=1779894315","url":"https:\/\/www.ardentbio.com\/products\/human-5-hydroxytryptamine-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}