{"product_id":"human-aqp-4-aquaporin-4-elisa-kit-5202026","title":"Human AQP-4 (Aquaporin-4) ELISA Kit","description":"\u003cp\u003eThe Human AQP-4 (Aquaporin-4) ELISA Kit measures AQP4 in Human Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti AQP-4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with AQP-4 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of AQP-4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49021132407012,"sku":"EH0967","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EH0967_c8c9e9c2-f55d-467e-92f7-368540198920.jpg?v=1779894781","url":"https:\/\/www.ardentbio.com\/products\/human-aqp-4-aquaporin-4-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}