{"product_id":"human-beta-actin-beta-actin-elisa-kit-5202026","title":"Human Beta-actin (Beta actin) ELISA Kit","description":"\u003cp\u003eThe Human Beta-actin (Beta actin) ELISA Kit measures Beta-actin in Human Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti β-actin antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with β-actin bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of β-actin in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49021176709348,"sku":"EH4017","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EH4017_efc922eb-d54a-4d39-8598-095e78017166.jpg?v=1779894951","url":"https:\/\/www.ardentbio.com\/products\/human-beta-actin-beta-actin-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}