{"product_id":"human-betaep-beta-endorphin-elisa-kit-5202026","title":"Human BETAEP (Beta-endorphin) ELISA Kit","description":"\u003cp\u003eThe Human BETAEP (Beta-endorphin) ELISA Kit measures Beta-EP in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with β-EP. β-EP in the sample or standard competes with a fixed amount of biotin-labeled β-EP for sites on a pre-coated antibody specific to β-EP. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of β-EP in the sample. The concentration of the target substance is inversely proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49021177299172,"sku":"EH0696","price":455.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EH0696_83607fad-fb69-421c-a171-306dbf47fdf0.jpg?v=1779894955","url":"https:\/\/www.ardentbio.com\/products\/human-betaep-beta-endorphin-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}