{"product_id":"human-dhea-s-dehydroepiandrosterone-sulfate-elisa-kit-5202026","title":"Human DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit","description":"\u003cp\u003eThe Human DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit measures DHEA-S in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with DHEA-S. DHEA-S in the sample or standard competes with a fixed amount of biotin-labeled DHEA-S for sites on a pre-coated antibody specific to DHEA-S. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of DHEA-S in the sample. The concentration of the target substance is inversely proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49021445079268,"sku":"EH2946-CM","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EH2946-CM_8188ed44-a0aa-4062-a144-6771a45764f3.jpg?v=1779895941","url":"https:\/\/www.ardentbio.com\/products\/human-dhea-s-dehydroepiandrosterone-sulfate-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}