{"product_id":"human-eg-vegf-endocrine-gland-derived-vascular-endothelial-growth-factor-elisa-kit-5202026","title":"Human EG-VEGF (Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit","description":"\u003cp\u003eThe Human EG-VEGF (Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit measures EG-VEGF in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti EG-VEGF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with EG-VEGF bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of EG-VEGF in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49021488890084,"sku":"EH0117","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EH0117_8de1f3f3-f68a-4db5-9295-b8af9009b5f6.jpg?v=1779896080","url":"https:\/\/www.ardentbio.com\/products\/human-eg-vegf-endocrine-gland-derived-vascular-endothelial-growth-factor-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}