{"product_id":"human-fcgammar-i-receptor-i-for-the-fc-region-of-immunoglobulin-g-elisa-kit-5202026","title":"Human FcGammaR I (Receptor I for the Fc Region of Immunoglobulin G) ELISA Kit","description":"\u003cp\u003eThe Human FcGammaR I (Receptor I for the Fc Region of Immunoglobulin G) ELISA Kit measures FCGR1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti FcγR I antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with FcγR I bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of FcγR I in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49021569401060,"sku":"EH3046","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EH3046_679dc115-6d1f-4cc2-9aba-b20a32050df9.jpg?v=1779896287","url":"https:\/\/www.ardentbio.com\/products\/human-fcgammar-i-receptor-i-for-the-fc-region-of-immunoglobulin-g-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}