{"product_id":"human-hla-drb1-hla-class-ii-histocompatibility-antigen-drb1-beta-chain-elisa-kit-5202026","title":"Human HLA-DRB1 (HLA class II histocompatibility antigen, DRB1 beta chain) ELISA kit","description":"\u003cp\u003eThe Human HLA-DRB1 (HLA class II histocompatibility antigen, DRB1 beta chain) ELISA kit measures HLA-DRB1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HLA-DRB1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with HLA-DRB1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of HLA-DRB1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49021883482340,"sku":"EH5188","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EH5188_fa4f957f-dbe7-426c-9b8d-4b78f3e13cf1.jpg?v=1779896988","url":"https:\/\/www.ardentbio.com\/products\/human-hla-drb1-hla-class-ii-histocompatibility-antigen-drb1-beta-chain-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}