{"product_id":"human-nos1-nnos-nitric-oxide-synthase-brain-elisa-kit-5202026","title":"Human NOS1\/NNOS (Nitric oxide synthase, brain) ELISA Kit","description":"\u003cp\u003eThe Human NOS1\/NNOS (Nitric oxide synthase, brain) ELISA Kit measures NOS1\/nNOS in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NOS1\/nNOS antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with NOS1\/nNOS bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of NOS1\/nNOS in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49022734991588,"sku":"EH0547","price":455.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EH0547_60b09b39-a5e3-4ea7-91f8-3b88be03adc1.jpg?v=1779898322","url":"https:\/\/www.ardentbio.com\/products\/human-nos1-nnos-nitric-oxide-synthase-brain-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}