{"product_id":"human-nos3-enos-nitric-oxide-synthase-endothelial-elisa-kit-5202026","title":"Human NOS3\/ENOS (Nitric oxide synthase, endothelial) ELISA Kit","description":"\u003cp\u003eThe Human NOS3\/ENOS (Nitric oxide synthase, endothelial) ELISA Kit measures NOS3\/eNOS in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NOS3\/eNOS antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with NOS3\/eNOS bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of NOS3\/eNOS in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49022735188196,"sku":"EH0554","price":455.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EH0554_0723a05b-3975-40a0-b6f0-0427484f573a.jpg?v=1779898325","url":"https:\/\/www.ardentbio.com\/products\/human-nos3-enos-nitric-oxide-synthase-endothelial-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}