{"product_id":"human-rnf-111-e3-ubiquitin-protein-ligase-arkadia-elisa-kit-5202026","title":"Human RNF-111 (E3 ubiquitin-protein ligase Arkadia) ELISA Kit","description":"\u003cp\u003eThe Human RNF-111 (E3 ubiquitin-protein ligase Arkadia) ELISA Kit measures RNF-111 in Human Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti RNF-111 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with RNF-111 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of RNF-111 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49023340675300,"sku":"EH4530","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EH4530_2a0e2c4c-e7e0-4223-95e9-166dfb4cfed0.jpg?v=1779899325","url":"https:\/\/www.ardentbio.com\/products\/human-rnf-111-e3-ubiquitin-protein-ligase-arkadia-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}