{"product_id":"human-sema4f-semaphorin-4f-elisa-kit-51126","title":"Human SEMA4F (Semaphorin 4F) ELISA Kit","description":"\u003cp\u003eThe Human (SEMA4F) Semaphorin 4F ELISA Kit measures Semaphorin 4F in samples. The plate has been pre-coated with Human SEMA4F antibody. SEMA4F present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA4F Antibody is added and binds to SEMA4F in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA4F antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SEMA4F. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.\u003c\/p\u003e","brand":"Bioassay Technology Laboratory","offers":[{"title":"96 wells","offer_id":48936688976100,"sku":"E1565Hu","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/E1565Hu.jpg?v=1779148605","url":"https:\/\/www.ardentbio.com\/products\/human-sema4f-semaphorin-4f-elisa-kit-51126","provider":"Ardent Bio LLC","version":"1.0","type":"link"}