{"product_id":"human-tpab-treponema-pallidum-antibody-elisa-kit-5202026","title":"Human TPAB (Treponema pallidum antibody) ELISA Kit","description":"\u003cp\u003eThe Human TPAB (Treponema pallidum antibody) ELISA Kit measures TP-Ab in Human Serum, Plasma. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with TP-Ab bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TP-Ab in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49024609943780,"sku":"EH4102","price":455.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/finetestelisakit_4178e943-c9c7-4879-8d8f-755a2dad4c2c.jpg?v=1779915521","url":"https:\/\/www.ardentbio.com\/products\/human-tpab-treponema-pallidum-antibody-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}