{"product_id":"monkey-sp-d-sftpd-pulmonary-surfactant-associated-protein-d-elisa-kit-5202026","title":"Monkey SP-D\/SFTPD (Pulmonary Surfactant Associated Protein D) ELISA Kit","description":"\u003cp\u003eThe Monkey SP-D\/SFTPD (Pulmonary Surfactant Associated Protein D) ELISA Kit measures SP-D\/SFTPD in Monkey Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SP-D\/SFTPD antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with SP-D\/SFTPD bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of SP-D\/SFTPD in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49025532920036,"sku":"EMK0363","price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EMK0363_91a53d4b-43e1-48a1-9e41-7e940ddab30f.jpg?v=1779901339","url":"https:\/\/www.ardentbio.com\/products\/monkey-sp-d-sftpd-pulmonary-surfactant-associated-protein-d-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}