{"product_id":"mouse-h2-aa-h-2-class-ii-histocompatibility-antigen-a-b-alpha-chain-elisa-kit-5202026","title":"Mouse H2-Aa (H-2 class II histocompatibility antigen, A-B alpha chain) ELISA Kit","description":"\u003cp\u003eThe Mouse H2-Aa (H-2 class II histocompatibility antigen, A-B alpha chain) ELISA Kit measures H2-Aa in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti H2-Aa antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with H2-Aa bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of H2-Aa in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49026619736292,"sku":"EM0643","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EM0643_46430e26-ed77-4cd5-b6b3-39bd34c2c014.jpg?v=1779902487","url":"https:\/\/www.ardentbio.com\/products\/mouse-h2-aa-h-2-class-ii-histocompatibility-antigen-a-b-alpha-chain-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}