{"product_id":"porcine-tnfsf13-april-tumor-necrosis-factor-ligand-superfamily-member-13-elisa-kit-5202026","title":"Porcine TNFSF13\/APRIL (Tumor necrosis factor ligand superfamily member 13) ELISA Kit","description":"\u003cp\u003eThe Porcine TNFSF13\/APRIL (Tumor necrosis factor ligand superfamily member 13) ELISA Kit measures TNFSF13\/APRIL in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TNFSF13\/APRIL antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with TNFSF13\/APRIL bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TNFSF13\/APRIL in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027374711012,"sku":"EP20009","price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EP20009_ce7f8df4-c9b9-49ad-be25-3b7e39d45a51.jpg?v=1779904358","url":"https:\/\/www.ardentbio.com\/products\/porcine-tnfsf13-april-tumor-necrosis-factor-ligand-superfamily-member-13-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}