{"product_id":"porcine-wisp1-ccn4-wnt1-inducible-signaling-pathway-protein-1-elisa-kit-5202026","title":"Porcine WISP1\/CCN4 (WNT1-inducible-signaling pathway protein 1) ELISA Kit","description":"\u003cp\u003eThe Porcine WISP1\/CCN4 (WNT1-inducible-signaling pathway protein 1) ELISA Kit measures WISP1\/CCN4 in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti WISP1\/CCN4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with WISP1\/CCN4 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of WISP1\/CCN4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027376283876,"sku":"EP0390","price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/EP0390_6e0c9ea6-23d7-4a2a-b9d5-02f4023b6fd4.jpg?v=1779904381","url":"https:\/\/www.ardentbio.com\/products\/porcine-wisp1-ccn4-wnt1-inducible-signaling-pathway-protein-1-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}