{"product_id":"rabbit-cd40l-tnfsf5-cluster-of-differentiation-40-ligand-elisa-kit-5202026","title":"Rabbit CD40L\/TNFSF5 (Cluster of Differentiation 40 Ligand) ELISA Kit","description":"\u003cp\u003eThe Rabbit CD40L\/TNFSF5 (Cluster of Differentiation 40 Ligand) ELISA Kit measures CD40L\/TNFSF5 in Rabbit Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CD40L\/TNFSF5 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with CD40L\/TNFSF5 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CD40L\/TNFSF5 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027378184420,"sku":"ERB0026","price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/ERB0026_13fdbcb6-4c36-41dc-b4c7-3adc729ad7d5.jpg?v=1779904428","url":"https:\/\/www.ardentbio.com\/products\/rabbit-cd40l-tnfsf5-cluster-of-differentiation-40-ligand-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}