{"product_id":"rat-5-hete-5-hydroxyeicosatetraenoic-acid-elisa-kit-5202026","title":"Rat 5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit","description":"\u003cp\u003eThe Rat 5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit measures 5-HETE in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 5-HETE. 5-HETE in the sample or standard competes with a fixed amount of biotin-labeled 5-HETE for sites on a pre-coated antibody specific to 5-HETE. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of 5-HETE in the sample. The concentration of the target substance is inversely proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027390865636,"sku":"ER0197","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/ER0197_b7a81820-92b8-40a3-92f5-9be150c77505.jpg?v=1779904657","url":"https:\/\/www.ardentbio.com\/products\/rat-5-hete-5-hydroxyeicosatetraenoic-acid-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}