{"product_id":"rat-5-lo-arachidonate-5-lipoxygenase-elisa-kit-5202026","title":"Rat 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit","description":"\u003cp\u003eThe Rat 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit measures Alox5 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti 5-LO antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with 5-LO bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of 5-LO in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027390963940,"sku":"ER0199","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/ER0199_6a19aba3-1f3b-4064-8fef-e2cd5f397ac0.jpg?v=1779904659","url":"https:\/\/www.ardentbio.com\/products\/rat-5-lo-arachidonate-5-lipoxygenase-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}