{"product_id":"rat-bfgf-fgf2-basic-fibroblast-growth-factor-elisa-kit-5202026","title":"Rat BFGF\/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit","description":"\u003cp\u003eThe Rat BFGF\/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit measures bFGF\/FGF2 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti bFGF\/FGF2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with bFGF\/FGF2 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of bFGF\/FGF2 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027440705764,"sku":"ER0764","price":455.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/ER0764_949eb490-62d5-4b8b-bb12-2b06f568ebcb.jpg?v=1779904948","url":"https:\/\/www.ardentbio.com\/products\/rat-bfgf-fgf2-basic-fibroblast-growth-factor-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}