{"product_id":"rat-bmp-2-bone-morphogenetic-protein-2-elisa-kit-5202026","title":"Rat BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit","description":"\u003cp\u003eThe Rat BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit measures BMP-2 in Rat Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti BMP-2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with BMP-2 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of BMP-2 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027446079716,"sku":"ER0010","price":455.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/ER0010_f85637fb-19b1-4404-82db-3fe2f0b7b6a4.jpg?v=1779904957","url":"https:\/\/www.ardentbio.com\/products\/rat-bmp-2-bone-morphogenetic-protein-2-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}