{"product_id":"rat-ck-18-m30-caspase-cleaved-keratin-18-elisa-kit-5202026","title":"Rat CK-18\/M30 (caspase-cleaved Keratin-18) ELISA Kit","description":"\u003cp\u003eThe Rat CK-18\/M30 (caspase-cleaved Keratin-18) ELISA Kit measures CK-18\/M30 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CK-18\/M30 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with CK-18\/M30 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CK-18\/M30 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027572170980,"sku":"ER1745","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/ER1745_5fbf28de-a536-4ef3-99eb-47a9b75c3b0a.jpg?v=1779905137","url":"https:\/\/www.ardentbio.com\/products\/rat-ck-18-m30-caspase-cleaved-keratin-18-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}