{"product_id":"rat-ck-18-m65-cytokeratin-18-elisa-kit-5202026","title":"Rat CK-18\/M65 (Cytokeratin 18) ELISA Kit","description":"\u003cp\u003eThe Rat CK-18\/M65 (Cytokeratin 18) ELISA Kit measures CK-18\/M65 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CK-18\/M65 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with CK-18\/M65 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CK-18\/M65 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027572203748,"sku":"ER1746","price":520.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/ER1746_ad566d30-a226-4512-99f2-6874c177d69c.jpg?v=1779905138","url":"https:\/\/www.ardentbio.com\/products\/rat-ck-18-m65-cytokeratin-18-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}