{"product_id":"rat-kim-1-kidney-injury-molecule-1-elisa-kit-5202026","title":"Rat KIM-1 (Kidney Injury Molecule 1) ELISA Kit","description":"\u003cp\u003eThe Rat KIM-1 (Kidney Injury Molecule 1) ELISA Kit measures Kim-1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Kim-1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Kim-1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Kim-1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027846242532,"sku":"ER0043","price":455.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/ER0043_51df7f37-253f-4088-a5ef-bd6d6b469e54.jpg?v=1779905935","url":"https:\/\/www.ardentbio.com\/products\/rat-kim-1-kidney-injury-molecule-1-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}