{"product_id":"rat-mip-1alpha-ccl3-macrophage-inflammatory-protein-1-alpha-elisa-kit-5202026","title":"Rat MIP-1Alpha\/CCL3 (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit","description":"\u003cp\u003eThe Rat MIP-1Alpha\/CCL3 (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit measures MIP-1Alpha in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti MIP-1α\/CCL3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with MIP-1α\/CCL3 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of MIP-1α\/CCL3 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"96 wells","offer_id":49027853549796,"sku":"ER0049","price":455.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/ER0049_2b6a1973-29f4-4fdc-bde9-4c32b8e2beb1.jpg?v=1779906053","url":"https:\/\/www.ardentbio.com\/products\/rat-mip-1alpha-ccl3-macrophage-inflammatory-protein-1-alpha-elisa-kit-5202026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}