{"product_id":"rat-pacap-27-pituitary-adenylate-cyclase-activating-polypeptide-27-elisa-kit-6072026","title":"Rat PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit","description":"\u003cp\u003eThe Rat PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat PACAP-27. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Rat PACAP-27. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PACAP-27 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e","brand":"Reed Biotech","offers":[{"title":"96 wells","offer_id":49120504119524,"sku":"RE2462R","price":450.0,"currency_code":"USD","in_stock":true},{"title":"48 wells","offer_id":49120504152292,"sku":"RE2462R-48w","price":315.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/ReedBiotechELISA.jpg?v=1765812936","url":"https:\/\/www.ardentbio.com\/products\/rat-pacap-27-pituitary-adenylate-cyclase-activating-polypeptide-27-elisa-kit-6072026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}