{"product_id":"sp-substance-p-elisa-kitkit-6072026","title":"SP (Substance P) ELISA KitKit","description":"\u003cp\u003eThe SP (Substance P) ELISA kitkit This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SP. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to SP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SP in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e","brand":"Reed Biotech","offers":[{"title":"96 wells","offer_id":49121088897252,"sku":"RE10009","price":450.0,"currency_code":"USD","in_stock":true},{"title":"48 wells","offer_id":49121088930020,"sku":"RE10009-48w","price":315.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/6238\/8964\/files\/RE10009-b-1.jpg?v=1781126140","url":"https:\/\/www.ardentbio.com\/products\/sp-substance-p-elisa-kitkit-6072026","provider":"Ardent Bio LLC","version":"1.0","type":"link"}