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Human AFM (Afamin) ELISA Kit
Human AFM (Afamin) ELISA Kit
The Human (AFM) Afamin ELISA Kit measures Afamin in samples. The plate has been pre-coated with Human AFAMIN antibody. AFAMIN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human AFAMIN Antibody is added and binds to AFAMIN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AFAMIN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human AFAMIN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3471Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
2.38 ng/mL
Detection Range
5-600 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
Functions as carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B (PubMed: 26902720). Binds vitamin E (PubMed: 15952736, PubMed: 12463752). May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient (PubMed: 15952736). May be involved in the transport of vitamin E across the blood-brain barrier (PubMed: 19046407). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Afamin
Regulatory Status
For Research Use Only
