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Human AKR1C3 (Aldo-Keto Reductase Family 1 Member C3) ELISA Kit
Human AKR1C3 (Aldo-Keto Reductase Family 1 Member C3) ELISA Kit
The Human (AKR1C3) Aldo-Keto Reductase Family 1 Member C3 ELISA Kit measures Aldo-keto reductase family 1 member C3 in samples. The plate has been pre-coated with Human AKR1C3 antibody. AKR1C3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human AKR1C3 Antibody is added and binds to AKR1C3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AKR1C3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human AKR1C3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3275Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
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2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
8.32 ng/L
Detection Range
15-3000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone (PubMed:10622721, PubMed:11165022, PubMed:7650035, PubMed:9415401, PubMed:9927279).
Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH (PubMed:14672942, PubMed:11165022).
Acts preferentially as a 17-ketosteroid reductase and has the highest catalytic efficiency of the AKR1C enzyme for the reduction of delta4-androstenedione to form testosterone (PubMed:20036328).
Reduces prostaglandin (PG) D2 to 11beta-prostaglandin F2, progesterone to 20alpha-hydroxyprogesterone and estrone to 17beta-estradiol (PubMed:15047184, PubMed:20036328, PubMed:10622721, PubMed:11165022, PubMed:10998348, PubMed:19010934).
Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol) (PubMed:10998348, PubMed:14672942, PubMed:11165022, PubMed:7650035, PubMed:9415401, PubMed:10557352).
Displays also retinaldehyde reductase activity toward 9-cis-retinal (PubMed:21851338). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Aldo-keto reductase family 1 member C3
Regulatory Status
For Research Use Only
