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Human ALOX5AP (Arachidonate 5-lipoxygenase-activating Protein) ELISA Kit

Human ALOX5AP (Arachidonate 5-lipoxygenase-activating Protein) ELISA Kit

The Human (ALOX5AP) Arachidonate 5-lipoxygenase-activating Protein ELISA Kit measures Arachidonate 5-Lipoxygenase-Activating Protein in samples. The plate has been pre-coated with Human ALOX5AP antibody. ALOX5AP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ALOX5AP Antibody is added and binds to ALOX5AP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ALOX5AP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ALOX5AP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E4982Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 1.41 ng/L
Detection Range 3-900 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Arachidonate 5-Lipoxygenase-Activating Protein
Regulatory Status For Research Use Only
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