1
/
of
1
Human Alpha-1-Antichymotrypsin (SERPINA3) ELISA Kit
Human Alpha-1-Antichymotrypsin (SERPINA3) ELISA Kit
This ELISA kit is designed to detect Human Alpha-1-Antichymotrypsin (Human SERPINA3). The assay plate has been pre-coated with mouse anti-Human SerpinA3 monoclonal antibody. When the sample containing SerpinA3 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human SerpinA3 Antibody is added to the wells and binds to SerpinA3 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human SerpinA3 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE064
Regular price
$624.00 USD
Regular price
$480.00 USD
Sale price
$624.00 USD
Unit price
/
per
2 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Human
Sensitivity
13.47 pg/mL
Detection Range
47-3000 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Cardiovascular, Cell Biology, Neuroscience
Background
SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a plasma alpha globulin glycoprotein, and is a member of serpin superfamily of the serine protease inhibitors consisting of at least 35 members. SerpinA3 has been demonstrated to inhibit the activity of certain serine proteases, such as cathepsin G found in neutrophils, and chymases present in mast cells, by inducing a major conformational rearrangement, and thus protects some tissues from damage caused by proteolytic enzymes. This enzyme is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease, and mutations of this gene have been observed in patients with Parkinson disease and chronic obstructive pulmonary disease. Besides, ACT gene polymorphism has been implicated with Alzheimer’s disease (AD), cerebral amyloid angiopathy (CAA), as well as stroke, since SerpinA3 is a major constituent of the plaques in AD and an inhibitor of amyloid beta peptide degradation.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Alpha-1-antichymotrypsin
Regulatory Status
For Research Use Only
