Human AMY1 (Alpha-amylase 1) ELISA Kit
Human AMY1 (Alpha-amylase 1) ELISA Kit
The Human AMY1 (Alpha-amylase 1) ELISA Kit measures AMY1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with anti AMY1 antibody. Sample or standard completely mixes with fixed amount of biotin-labeled AMY1 working solution and also add into each well. AMY1 in the sample or standard competes with biotin-labeled AMY1 to bind with antibody precoated on the microplate. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of AMY1 in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
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