The Human (ANP32A) Acidic Leucine-rich Nuclear Phosphoprotein 32 Family Member A ELISA Kit measures Acidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member A in samples. The plate has been pre-coated with Human ANP32A antibody. ANP32A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ANP32A Antibody is added and binds to ANP32A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ANP32A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ANP32A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundMultifunctional protein that is involved in the regulation of many processes including tumor suppression, apoptosis, cell cycle progression or transcription (PubMed: 16341127, PubMed: 11360199, PubMed: 18439902, PubMed: 10400610). Promotes apoptosis by favouring the activation of caspase-9/CASP9 and allowing apoptosome formation (PubMed: 18439902). In addition, plays a role in the modulation of histone acetylation and transcription as part of the INHAT (inhibitor of histone acetyltransferases) complex. Inhibits the histone-acetyltranferase activity of EP300/CREBBP (CREB-binding protein) and EP300/CREBBP-associated factor by histone masking (PubMed: 11830591). Preferentially binds to unmodified histone H3 and sterically inhibiting its acetylation and phosphorylation leading to cell growth inhibition (PubMed: 16341127). Participates in other biochemical processes such as regulation of mRNA nuclear-to-cytoplasmic translocation and stability by its association with ELAVL1 (Hu-antigen R) (PubMed: 18180367). Plays a role in E4F1-mediated transcriptional repression as well as inhibition of protein phosphatase 2A (PubMed: 15642345, PubMed: 17557114). (Microbial infection) Plays an essential role in influenza A, B and C viral genome replication (PubMed: 32694517, PubMed: 33045004, PubMed: 33208942, PubMed: 30666459). Mechanistically, mediates the assembly of the viral replicase asymmetric dimers composed of PB1, PB2 and PA via its N-terminal region (PubMed: 33208942). Plays also an essential role in foamy virus mRNA export from the nucleus (PubMed: 21159877). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteAcidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member A
