Human Anti-CoL (corpus luteum) antibody ELISA Kit
Human Anti-CoL (corpus luteum) antibody ELISA Kit
The Human Anti-CoL (corpus luteum) antibody ELISA Kit measures CoL-Ab in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen-Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antigen, then it binds with CoL-Ab bound to precoated antigen. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CoL-Ab in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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