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Human APAF-1 (Apoptosis Protease Activating Factor-1) ELISA Kit
Human APAF-1 (Apoptosis Protease Activating Factor-1) ELISA Kit
The Human (APAF-1) Apoptosis Protease Activating Factor-1 ELISA Kit measures apoptosis protease activating factor-1 in samples. The plate has been pre-coated with Human Apaf-1 antibody. Apaf-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human Apaf-1 Antibody is added and binds to Apaf-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Apaf-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human Apaf-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1835Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
8.14 ng/L
Detection Range
15-3000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cell Biology, Apoptosis, Enzyme & Kinase
Background
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
apoptosis protease activating factor-1
Regulatory Status
For Research Use Only
