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Human ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) ELISA Kit
Human ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) ELISA Kit
The Human (ARNT) Aryl Hydrocarbon Receptor Nuclear Translocator ELISA Kit measures Aryl Hydrocarbon Receptor Nuclear Translocator in samples. The plate has been pre-coated with Human ARNT antibody. ARNT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ARNT Antibody is added and binds to ARNT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ARNT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ARNT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2501Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
7.86 ng/L
Detection Range
15-3000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Neuroscience
Background
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity).
The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Aryl Hydrocarbon Receptor Nuclear Translocator
Regulatory Status
For Research Use Only
