This ELISA kit is designed to detect Human Aromatic-L-Amino-Acid Decarboxylase (Human DDC). The assay plate has been pre-coated with mouse anti-Human DOPA Decarboxylase monoclonal antibody. When the sample containing DOPA Decarboxylase is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human DOPA Decarboxylase Antibody is added to the wells and binds to DOPA Decarboxylase in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human DOPA Decarboxylase present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
BackgroundDopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
