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Human Azurocidin (AZU1) ELISA Kit

Human Azurocidin (AZU1) ELISA Kit

This ELISA kit is designed to detect Human Azurocidin (Human AZU1). The assay plate has been pre-coated with mouse anti-Human Azurocidin monoclonal antibody. When the sample containing Azurocidin is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human Azurocidin Antibody is added to the wells and binds to Azurocidin in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human Azurocidin present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE116
Regular price $754.00 USD
Regular price $580.00 USD Sale price $754.00 USD
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Product Details

Species Reactivity Human
Sensitivity 1.4 pg/mL
Detection Range 6.25-400 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Cardiovascular, Immunology, Microbiology
Background Azurocidin (AZU1), also known as heparin-binding protein (HBP) or cationic antimicrobial protein 37 (CAP37), is an azurophil granule antibiotic protein, with monocyte chemotactic and antibacterial activity. The Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. Azurocidin is a member of the serine protease family that includes Cathepsin G, neutrophil elastase (NE), and proteinase 3 (PR3), however, Azurocidin is not a serine proteinase since the active site serine and histidine residues are replaced. Neutrophils arriving first at sites of inflammation release Azurocidin, which acts in a paracrine fashion on endothelial cells causing the development of intercellular gaps and allowing leukocyte extravasation. It thus be regarded as a reasonable therapeutic target for a variety of inflammatory disease conditions.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Azurocidin
Regulatory Status For Research Use Only
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