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Mouse Carboxypeptidase B1 ELISA Kit
Mouse Carboxypeptidase B1 ELISA Kit
This ELISA kit is designed to detect Mouse Carboxypeptidase B1. The assay plate has been pre-coated with mouse anti-Mouse Carboxypeptidase B1 monoclonal antibody. When the sample containing Carboxypeptidase B1 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Mouse Carboxypeptidase B1 Antibody is added to the wells and binds to Carboxypeptidase B1 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Mouse Carboxypeptidase B1 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE312
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
2.51 pg/mL
Detection Range
12.5-800 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Metabolism, Signal Transduction
Background
Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Carboxypeptidase B1
Regulatory Status
For Research Use Only
